NEWS LETTER    第10号 (2005年 秋)

    橋岡 豪人    はしおか たけと






 Conservation program to maintain parrot diversity
 -Sequence diversity of nuclear exon RAG-1 in parrots

     Dwi Astuti    ドゥイ・アストゥティ

  About 350 parrot species are distributed in the world. This group retains worldwide popularity, because they have several unique characteristics and economic value. The over-hunting has drastically depopulated the parrots, therefore, all parrot species are listed in CITES appendix. To keep parrot diversity, the conservation program is ungently requested. On the other hand, the information at molecular level in parrots remains poorly known. Therefore, we planned to conduct some research at the DNA level as a basic information to provide the conservation program and to solve tasonomic problems in parrots. This study is a part of our planning. As a part of biodiversity theme, therefore, COE profect of Hokkaido University supported this study.
  DNA genome was extracted from blood, feathers or tissue samples. Totally 639-bp of nuclear exon recombinant-activating gene (RAG-1) was amplified using PCR from some parrots in Indonesia. The RAG-1 exon gene of parrots was relatively rich in A-T. Average nucleotide composition was 30.8% of adenine, 26.3% of thymine, 21.3% of cytocine, and 21.7% of guanine. The intraspecific variation of the DNA sequence was so little that, even if there is, only one substitution was detected between individuals. The low sequence variation was also detected between species of genus Cacatua (White cockatoos), and between some genera of subfamily Loriinae (Lories and Lorikeets).